[SCT perfusion in the diagnosis of sellar and parasellar tumors].

UNLABELLED: The study is devoted to the use of a minimally invasive method of SCT perfusion for the differential diagnosis of sellar and pаrаsellar tumors. Given a wide differential diagnostic range of tumors occurring in this area, the tumor perfusion indicators were used as an auxiliary diagnostic criterion. MATERIAL AND METHODS: The study analyzed outcomes in 115 patients with various tumors of the sellar area who underwent surgery or biopsy for histological verification of the diagnosis. RESULTS: The statistically significant differences were obtained for certain groups of tumors based on the values of hemodynamic parameters (p<0.05) that enable, with a high confidence, making a conclusion on the histologic type of most tumors. CONCLUSION: These findings demonstrated that SCT perfusion is a highly informative method of the preoperative differential diagnosis of these tumors. The sensitivity and specificity of SCT perfusion in the study were 100% and 81.2%, respectively.

[Cloning, primary structure determination and expression of preproinsulin cDNA from human insulinoma in Escherichia coli]. / Klorirovanie, opredelenie pervichnoi struktury i ékspressiia kDNK preproinsulina iz insulinomy cheloveka v Escherichia coli.

A human insulinoma cDNA library was constructed in expression plasmid vector pUEX1. Clone pUEX1Ins12 was selected from human insulinoma cDNA library by means of hybridization with the insulin probe and a nucleotide sequence of the insertion was determined. It codes for full size amino acid sequence preproinsulin and furthermore, contains the entire 3'-end of noncoding mRNA region and 44 nucleotides from the 5'-untranslated region. The bacterial strain pUEX3Ins8 producing preproinsulin as beta-galactosidase fusion protein was constructed.